CEDB20003 Lecture Notes - Lecture 2: Haematoxylin, Photon, Hair Cell
13 Aug 2018
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Document Summary
Matches image sie to resolution limit of your eye key principle of the microscope bigger image doesn"t necessarily mean better if you cannot see the high resolution. Factors affecting resolution (d) father of modern optics. Worked out and designed the first microscope to get a small d you need a small wavelength which can be achieved using a filter. The higher the magnification of the objective, the higher the angular aperture how the life is bent through the condencer and the eye lower power lense (smaller lense) has a bigger working distance. The higher the refravtive index (n) the higher the resolution. We can see bacteria and cell nuclei and perhaps large vesicles and granules but we cannot resolve viruses or smaller intracellular organelles such as mitochondiria or ribosomes. Wavelength of an electron decreases with velocity at 100,000v wavelength = 0. 004nm, d= 0. 002 nm in practice, d=01nm for electron microscope compared to d=0. 2um for light microscope.
