BIOL1007 Lecture Notes - Lecture 3: Ethanol Precipitation, Electrophoresis, Hydrophile
14 Oct 2018
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All linear biopolymers have a defined beginning and end. Biopolymers are synthesized in one direction only increasing the backbone. Some of the monomer is lost in polymerization, leaving a (cid:862)residue(cid:863) in(cid:272)orporated in the growing chain. L01: identify the main chemical components of nucleic acids and their interactions involved in base pairing between nucleic acid strands. Describe how these properties can be exploited in experimental situations. Common, no matter what base is attached. Aromatic ring structures: flat/planar, absorb at approx. C and g complement each other (3 hydrogen bonds so stronger binding) A and t/u complement each other (2 hydrogen bonds so a bit weaker) Flat bases stack on top of each other. L02: distinguish dna from rna, and explain how these differences relate to their different roles. Bases are generally stable, except spontaneous deamination c>u. Not a good idea for dna (stable genetic information); uracil in dna is recognised as worng and repaired.
