400981 Lecture Notes - Lecture 18: Leukotriene B4 Receptor, Wee1, Protein Kinase
26 Feb 2020
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Document Summary
The protein kinase cdr2 which negatively regulates wee1 and the cdr2-related kinase cdr1 which directly phosphorylates and inhibits wee1 in vitro are localized to a band of cortical nodes in the middle of interphase cells. After entry into mitosis, cytokinesis factors such as myosin ii are recruited to similar nodes; these nodes eventually condense to form the cytokinetic ring. A previously uncharacterized protein, blt 1, was found to colocalize with cdr2 in the medial interphase nodes. Blt1 knockout cells had increased length at division, which is consistent with a delay in mitotic entry. This finding connects a physical location, a band of cortical nodes, with factors that have been shown to directly regulate mitotic entry, namely cdr1, cdr2, and blt1. Further experimentation with gfp -tagged proteins and mutant proteins indicates that the medial cortical nodes are formed by the ordered, cdr2-dependent assembly of multiple interacting proteins during interphase.
