IMED1002 Lecture Notes - Lecture 27: Western Blot, High Fidelity, Mutation
12 Jul 2018
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Mutations: be able to describe the different types of gene mutation at the dna and protein level, and understand how these might appear on a northern and western blot. Recall that dna is very stable, highly specificity of base pairing. Long term information storage with near perfect fidelity, so chemical changes are very slow, but mistakes can still happen> mutations and evolution. Mrna is very short lived> it carries code to make protein and is then degraded. Mistakes can occur at many levels: translation error-protein affected transcription error- mrna and protein affected. O(cid:374)ly affects that cell, (cid:858)(cid:373)utatio(cid:374)(cid:859) is (cid:374)ot i(cid:374)he(cid:396)ited self-replication- dna, rna and protein affected. If mutation occurs in dna of cell that will be an egg of sperm then the progeny will contain the mistake: translational or transcriptional mistakes would not have this issue. Only germ line mutations that will be passed on mutations.
