IMED1002 Lecture Notes - Lecture 6: Chromosome, Flap Structure-Specific Endonuclease 1, Dna Gyrase
15 Aug 2018
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Document Summary
Define the process and direction of dna replication and know it is confined to the s phase of the cell cycle. Recognise the functions of the dna pol alpha, delta and epsilon and pyrophosphatase. Have an understanding of the semi-conservative and high fidelity nature of dna replication. Understand the roles of helicase, rna primer, template strands, magnesium ions, dna ligase in replication. Be able to draw/label and recognise the differences between leading and lagging strand synthesis. Two strands of dna are joined by h-bonds and can be separated without breaking covalent bonds. Watson and crick, nature (vol 171) 1953. A in parental strand dictates t in daughter strand. T in parental strand dictates a in daughter strand. C in parental strand dictates g in daughter strand. G in parental strand dictates the c in daughter strand. Are going to discuss enzymes in eukaryotes with some simplifications for clarity.
