BIOL 2030 Lecture Notes - Lecture 23: Endonuclease, Ecori, Plasmid
16 Mar 2017
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Deals with modern technology and methods to study molecular biology. Answer: to separate dna molecules based on their molecular mass or size in the number of base pairs in a fragment of dna. If heated to 100c, it forms an initial gel: begins are sol state, after cooling it down from the initial gel, it forms a gel structure with pores. Formation is similar to jello - heat it up, and cool it down. Important that it is uncharged = it would not interact with the dna that goes through it. Parameters that do not influence the rate of migration of dna molecules during agarose gel- electrophoresis: %gc or sequence of a dna molecule. "ases" tells you you"re looking at an enzyme. Seuqnce of nucleotide bases reads the same on the top strand as the sequence of nucleotide bases reads on the bottom strand of the dna molecule in the 5" to 3" direction: palidromic sequences.
