BIOL 200 Lecture Notes - Lecture 18: Aequorea Victoria, Green Fluorescent Protein, Channelrhodopsin
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You take a protein, and fuse it with a tag (e. g. gtp) so that you could visualize it & study its function. The protein is cloned then tagged, so you can study the protein independent of its cell. When the protein-in-question goes through translation, it goes through it as a hybrid. Gtp-tagged histones and tubulin show how mitosis happens. Channelrhodopsin: membrane channels that use light energy to carry out a chemical change in the cell. Neuronal function is independent on ionic flux in/out of cells. You can manipulate channelrhodopsin and thus manipulate behavior. You can put the channelrhodopsins near a specific neuron, shine a light of a specific wavelength, activate and elicit a specific behavior. (recall: mouse eating cheese in cage; red/blue light on its head). You can also use genetic engineering to create calcium sensors that indicate neural activity. Molecular probes: allows you to find a needle in a haystack . e. g. dna in a.