BIOL 200 Lecture Notes - Lecture 8: Noncoding Dna, Trp Operon, Sanger Sequencing
Document Summary
The mist: the genome, eukaryotic gene structure, and. Sanger sequencing: use ddntps (dideoxy ntps; unable to elongate dna strand; every time a ddntp is added, the rxn terminates) So rxn terminates at different bases, identified by fluorescence. For an electrophoresis gel, read from bottom up, so template strand sequence (5" -> 3") = complementary to bases read from bottom up. The c-value enigma: the lack of correlation b/w genome size and the biological complexity of an organism. Diff. in genome size due to amt of non-coding dna. Gene density varies (among eukaryotes) due to size of intragenic (introns)& intergenic (b/w genes) regions. Gene = the entire nucleic acid sequence that"s necessary for the synthesis of a functional gene product (protein, or rna) Polycistronic rna: rna with many start sites for protein synthesis (translation) Rare in eukaryotes (e. g. trp gene in yeast) Poly-a site: 3" cleavage and polyadenylation site of rna transcript mrna spliced (introns out)
