BIOL 200 Lecture Notes - Lecture 28: Eif2, Ferritin, Ern1
Document Summary
More mrna = more protein (usually) mrna levels and protein levels that do not correlate => translational regulation. Recall: phosphorylation of eif2 prevents formation of the 43s pre-initiation complex, thus blocking translation (during starvation) Nutrient-poor conditions block translation since you can"t form that pre- ne. Nutrient-poor conditions block translation since you can"t form that pre- initiation complex (translation regulated by env. conditions) here you phosphorylate eif2 (remember that eif2-gtp is bound to meti in translation initiation) eif4e-bp blocks eif4e-eif4g interaction. Could also enhance translation by helping w/ the formation of scaffolds. Ire-bp (iron response element binding protein) is important in maintaining the iron conc. in the cell. They destabilize the tfr mrna. (has nothing to do with ire1) Has 2 conformations (that depends on conc. of fe) Low fe: takes on conformation that allows ire-bp to bind ires. This is b/c when iron levels are high, you don"t need to make more tfr; tfr brings more iron into the cell!
