BIOL 200 Lecture Notes - Lecture 4: Rna Polymerase Iii, Rna Polymerase Ii, Rna Polymerase I
10 Dec 2014
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Chromatography experiment explained: you take cells to observe rna polymerase activity in a specific cell type, grind it, mix it, you put it over a column and derive fractions. Test the fractions for rna polymerase activity: to test for rna polymerase activity: add rntp and wait for rna to form. Add the substrate then see if rna forms: black line denotes total protein and blue line shows rna in presence of alpha-amanitin. The red line shows the fractions that are able to show rna polymerase activity: alpha amanitin is a toxin produced by a mushroom. 3 kinds of rna polymerases in eukaryotes: rna polymerase i is found in the nucleolus and transcribes only precursor ribosomal. Rna (rrna: rna pol ii transcribes mrnas and four small nuclear rnas that take part in rna splicing. In bacteria there are 5 subunits, yeast has 12. Bacteria= prokaryotic yeast= eukaryotic: the cleft is where the polymerase interacts with the dna.
