BIOL 200 Lecture Notes - Lecture 32: Leucine, Petal, Sepal
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In each fraction you add the radioactive probe and set up a condition so any dna-binding protein can interact with that dna and if it does, it will form a ternary complex of the dna, the probe, and the. If protein is interacting with certain sequence, dna digest cannot cut: regions of dna protected by dna-binding protein against dna digest, can run sequencing gel beside and find exact sequence that is being protected. Transcription factors are modular (cid:120) most transcription factors have several domains that each perform distinct functions (cid:120) characteristics of dna-binding domains will dictate (cid:120) (cid:449)hi(cid:272)h se(cid:395)ue(cid:374)(cid:272)es i(cid:374) the dna they"ll i(cid:374)te(cid:396)a(cid:272)t (cid:449)ith. Just interacting with dna is insufficient to turn on transcription there are other functional regions within. Just binding dna is not enough to activate the transcription of a reporter gene (cid:120) you require a dna-binding domain coupled with a transcriptional activation domain. Helix-loops-helix proteins (cid:120) hlh proteins are very similar to leucine zipper proteins (cid:120)
