BIOL 200 Lecture Notes - Lecture 36: Pyrimidine, Ribonucleoprotein, Transesterification
22 Jun 2016
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If you have antibody on dna binding protein, take mechanically sheared mixture (1000-2000 bp) and mix antibody with mixture and antibody will bind to protein within its context. Protects from enzymatic degradation while facilitating nuclear export and recognition by translation initiation factors. Splice factors recognize modification at end and use it to splice pre-mrna o 2" hydroxyl of ribose group of first base (and second in vertebrates) methylated. Pre-mrna mrna: splicing: introns o must be spliced from eukaryotic genes prior to transcription o visualized by hybridization experiments. Mrna of adenovirus hexon gene hybridized to dna fragment containing hexon gene intron sequences loop out since no corresponding sequence in mrna o borders are highly conserved. Useful for in silico prediction of mrna sequences based on genomic sequence data. Snrna : pre-mrna pairing critical for u1 function in splicing: splicing o most introns spliced by spliceosome; consists of different steps that require particular spliceosome subunits o some introns can self-splice.
