ANAT 262 Lecture Notes - Lecture 7: Nuclear Lamina, Signal Recognition Particle, Nonsense-Mediated Decay

• School: McGill University
• Department: Anatomy & Cell Biology
• Course: ANAT 262
• Professor: John Presley

Document Summary

Nucleus: approximately size of rbc, 5 um to 10um. Organelle is a membrane-bound compartment that possesses a specific function. Organization of the nucleus (1) nuclear envelope (ne) Nuclear pore complexes: helps in macromolecules trafficking between cytoplasm and nucleus (2) nuclear lamina: under inner nuclear membrane, related in nuclear diseases (3) nucleus interior. Nuclear bodies: family of compartment identifies by immuno-staining, supply or process supplying. Nuclear matrix: defined experimentally by extracting nucleus using salt, detergent, Contains proteins providing scaffold (skeletal like) elements in nucleus, provides binding sites for transcription factors. Compartment that contains lots of different proteins (complex). One major task is generation of ribosomal subunits. Large subunits have 3 different subunits generated by initial transcript named 47s into 28s, 5. 8s and 5s ribosomal rna. 5s is transcribed in nucleus but outside nucleolus. Large ribosomal subunit contains 47 proteins, while small subunit (18s) has 32 proteins. Nucleoli have different forms (mostly spherical) and can have different sizes.