ANAT 262 Lecture Notes - Lecture 6: 3D Reconstruction, Cryogenic Transmission Electron Microscopy, Ice Crystals
15 Jul 2016
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Document Summary
Classical em of tissues and cells: morphology (function) and morphological changes associated with diseases. Immunogold labeling continues to a tool frequently and routinely used to localize proteins at high resolution. Protein mis-localization can be responsible for certain pathology. Em observation of infectious agents (bacteria and viruses) can provide new therapeutic directions. Em observation is a fast diagnostic tool (viruses have their characteristic morphology that allows to identify them easily) Tem images are at first approximation projection images. Easy to misinterpret tem images due to projection artefact. You get to serial sectioning since tem samples need to be thin. If two objects, you would see a space. 2 er: sheets of er which are connected via tubular structures which is the smooth er. We can stack the serial sectioning computationally, and we would see the tubular structures in 3d. 3d information is required to understand organelles organization => serial sections.
