BIOC 300D2 Lecture Notes - Lecture 2: Bio-Rad Laboratories, Coomassie Brilliant Blue, Glutathione

• School: McGill University
• Department: Biochemistry
• Course: BIOC 300D2
• Professor: Jose G.Teodoro

Document Summary

Codon: every 3 nucleotides code for one amino acid. Reading frame of any rna and dna is very important. Shift reading frame one nucleotide forward (2nd) or two nucleotide forward (3rd)=> you would produce a different polypeptide. Each reading frame would yield a different polypeptide. In cloning or when planning on expressing proteins, very important to think about the reading frame since you need to clone in-frame (right protein to be made) In almost all cases of protein synthesis, methionine is the first amino acid incorporated into the nascent polypeptide chain (encoded by aug, gug, auu) Note that gug and auu encodes the aa valine and isoleucine. Shine-dalgarno as start codon, they will encode met. Major step in translation is translation initiation: major complex in charge of initiation when capping is done (eif4f): eif4e, eif4g and eif4a. Formation of initiation complex is a rate-limiting step.