NUR1 311 Lecture Notes - Lecture 4: Tetanus, Bordetella, Toilet Seat
8 Dec 2017
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Identifying microorganisms in the laboratory: various microorganisms are identified in the microbiology labs, including: bacteria, fungi, parasites, and viruses. Prions are usually sent out to special referral labs because they are very difficult to identify: different techniques for identification of microorganisms exist: Detection of antigens, if they can"t be grown (strep group a) Molecular testing involves looking at dna and rna of bacteria, newer method (vre, mrsa). Serological testing involves looking at antibodies (hepatitis) Identification of bacteria: bacteria identification involves plating onto blood agar plates, and growing them in enriched broths or selective media. Using certain media will encourage growth of certain bacteria over others. Not all bacteria can be stained depends on the cell wall. If stat report is needed specimen is stained before plating. Medically important bacteria will be stained differently depending on the properties of the cell wall (thickness). All bacteria have different cell walls and cell membranes so they are stained differently: gram stain.
