BIOCHEM 2EE3 Lecture Notes - Lecture 3: Missense Mutation, Ribosomal Rna, Hydrolysis

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Multicellular organisms have larger genomes than those of bacteria or viruses: however, there is no simply relationship between genome size and organismal complexity. Human genome consists of about three billion base pairs. Eukaryotic genomes must contain considerable dna that does not code for proteins or for the rna machinery of protein synthesis. Most eukaryotes require and have much larger genomes than prokaryotes. Difference between bacterial and eukaryotic genomes is the diploid nature of eukaryotic genomes. Physical organization of eukaryotic genes: chromosomes and chromatin. Mrna must be exported from the nucleus for translation the primary transcript undergoes post-transcriptional processing before it is exported: the multiple euk chromosomes are contained within the nucleus, except during mitosis when the nuclear envelope disintegrates. Chromatin: the most abundant proteins in chromatin are a set of five highly conserved, low molecular-weight, strongly basic proteins- histones, the chromatin of euks consists of dna complexed with histones and non-histone proteins.

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