BIOCHEM 3H03 Lecture Notes - Lecture 1: Oxalate, Ethylenediaminetetraacetic Acid, Blood Sugar
18 Jan 2017
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Laboratory medicine: provides lab results, interpretation, and consultation, examines chemical content of biological fluids, needs: accuracy, relevance, and be timely, guides decision making and treatment. Steps to lab medicine: diagnosis: define the reason for signs, symptoms or complaints, monitor: follow the course of the condidtion, screening: detect disease when no symptoms occur. Can be population-based or risk-based: prognosis: determine the timeline for a future event. Choice depends upon: the analyte, biological considerations, and easiness to collect. Testing can be done on: whole blood, serum, and plasma. Ex: whole blood is the quickest as no clotting or centrifuge is required. Serum = whole blood cells coagulation factors: blood samples naturally coagulate. Anti-coagulants used: heparin: inhibits activity of thrombin (by promoting anti-thrombin *out of scope of course, oxalate: binds calcium ion needed for coagulation, edta: chelates calcium ion, fluourine: inhibits glycolysis = more accurate measurement of blood glucose content. If you add nothing to blood: clotting occurs.
