BIOLOGY 1A03 Lecture Notes - Lecture 3: Hek 293 Cells, Cd14, Plasmid

• School: McMaster University
• Department: Biology
• Course: BIOLOGY 1A03
• Professor: Lovaye Kajiura

Document Summary

Main point: opsonins are not required to clear s. pneumoniae. Opsonins, which are any molecule that enhances phagocytosis by marking an antigen for an immune response, such as complement, are not required for clearing s. pneumoniae bacterium as it was shown in mice. Essentially, s. pneumoniae was inserted into the mice that did not have mac-1 or c3 components, which are important complement opsonins, through the nasal cavity. Afterwards the amount of bacterium in the mice"s nasal cavity was measured separately in the. Wt, mouse that lacked c3 and mouse that lacked mac-1. Main point: marco is associated with the clearance of s. pneumoniae. Results showed that by day 21, the. S. pneumoniae whereas the wt mouse w/ marco had already done so showing the importance of macro in clearance of s. pneumoniae. Main point: the cell population that expresses marco are resident cells, not effector cells.