BIOLOGY 1A03 Lecture Notes - Lecture 4: Exon Skipping, Start Codon, Molecular Machine

• School: McMaster University
• Department: Biology
• Course: BIOLOGY 1A03
• Professor: Alastair Tracey Rosa Da Silva

Document Summary

Biology 1a03; applied lecture (wednesday, september 27th 2017) In processing, you want to splice out the introns. Note; 5" prime region, before the translation start codon, you have a utr (a non translated region) Splicing is catalyzed by an enzyme called the spliceosome, it recognizes specific sequences in the introns, they splice out the introns to give you the mature mrna, in order to produce functional proteins. A spliceosome; a molecular machine containing rnas and proteins. Intron retention (likely with premature stop codons); extra sequences. Exon skipping, intron retention (would likely create early stop codons) The experiments a piece of double stranded dna encoding a protein was separated into single strands and is hybridized (allowed to bind) with the mature mrna for the encoded protein: interpretation of micrograph. Single-stranded dna only, single stranded dna base paired with mrna. Muscle cells depend on stimulation; muscles cells weaker or die.