BIOLOGY 1A03 Lecture Notes - Lecture 15: Reading Frame, Hbb
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BIOLOGY 1A03 Full Course Notes
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The ability to amplify dna in a tube with the pcr technique was developed by kary mullis: Allows for the copying of millions of dna from very small initial samples. She light on diagnosis of genetic defects, detection of viral dna in cells, producing large amounts of dna from fossils containing trace amounts of dna and can link individuals to. Sample dna is placed into a tube with buffered solution with essential ion and salts and short single-stranded dna primers (15-30 nucleotides) that bind to the dna template. Free dntps (deoxyribonucleotides) are added to the tube to help the replication process. Pcr does not need many enzymes to unwind the helix, instead they are put in a thermocycler machine that goes through phases of heating and cooling to help the dna replication process. Each cycle of replication during a pcr reaction includes 3 stages: