BIOLOGY 1A03 Lecture Notes - Lecture 17: Thermus Aquaticus, Kary Mullis, Agarose Gel Electrophoresis
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BIOLOGY 1A03 Full Course Notes
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The ability to amplify dna in a tube with the pcr technique was developed by kary mullis: Allows for the copying of millions of dna from very small iniial samples. She light on diagnosis of geneic defects, detecion of viral dna in cells, producing large amounts of dna from fossils containing trace amounts of dna and can link individuals to dna samples during invesigaions. Sample dna is placed into a tube with bufered soluion with essenial ion and salts and short single-stranded. Dna primers (15-30 nucleoides) that bind to the dna template. Free dntps (deoxyribonucleoides) are added to the tube to help the replicaion process. Pcr does not need many enzymes to unwind the helix, instead they are put in a thermocycler machine that goes through phases of heaing and cooling to help the dna replicaion process. Thermus aquaicus that are adapted to live in hot springs with temperatures above 95 oc.