BIOLOGY 2A03 Lecture Notes - Lecture 2: Uncoupling Protein, Intermembrane Space, Oxidative Phosphorylation

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4 May 2017

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All the various processes (chemical reactions)that occur in the cell. Hydrolysis (water added) and condensation reactions (water removed) Phosphorylation of proteins phosphate group is taken from or added to atp. Redox reactions - key in the electron transport chain. Reactions occur to establish an equilibrium at the completion. Activation energy - reactants and products have energy . Barrier between reactants to products - energy must be added to overcome it. Even if products have lower energy compared to the reactants. Enzymes reduce the activation energy by a large amount. Many reactions wont occur without enzymes at all. Enzymes aren"t altered in reactions, substrate specific (this is important to the proteins). Substrate fits into enzyme (reactant) and facilitates the reaction. Once enzyme catalyzes reaction, product is released and this continues. Rate of reaction as a function of substrate, as substrate increases, the rate of reaction also increases. More substrate - tends to be a threshold and saturate the enzyme.

Related Questions

Oxidation reduction reactions are coupled and referred to as redox reactions. Electrons are the atomic particles that participate in chemical bonds and are used by cells to generate an electrochemical gradient across membranes. Cells have require ATP to maintain membrane properties and metabolism, because ATP has high energy phosphate bonds that may be cleaved to release energy. Cells synthesize ATP through phosphorylation. Cells must perform many chemical reactions within a certain temperature, pH and osmolarity range. In general chemical reactions in a cell would occur over time without enzymes, and it might require big changes in temperature. Enzymes are organic catalysts that increase the rate of reactions without being altered in the process. Review protein structure and function in chapter 2 to understand how enzymes work. Enzymes also may act as 'switches' and be the rate controlling steps in a biochemcial pathway. There are several ways enzyme activity is altered by other factors or physical conditions.

1. The bacterial enzyme streptokinase is used to digest fibrin in patients with atherosclerosis. Why doesn't the injection of streptokinase cause a streptococcal infection?

2. How do we know that streptokinase will break down fibrin and not functional tissue?

3. 5 bromouracil is used as an anti cancer drug it is mistaken for thymine by cellular enzymes during DNA replication, name the enzyme site the nucleoside analog is probably binding.

Most of a cell's energy is derived from the oxidation of carbohydrates. Glucose is the most common oxidized carbohydrate. There are two types of glucose catabolism respiration and fermentation.

4. Is glucose completely broken down in respiration or fermentation?

greenhedgehog909

1.When something appears blue, it is absorbing all colors except__________.

yellow

blue

red

green

orange

2.During heavy exercise, lactic acid can build up in ourmuscles. Why? How does this resemble the metabolism of bacteria andyeast?

lactic acid is results from insufficient oxygen during sugarmetabolism; it is fermentation

lactic acid accumulates when we are dehydrated; bacteria andyeast use the same pathway

lactic acid is built up from carbon dioxide; bacteria and yeaststore energy in this way

3.Why must a cell keep a similar concentration of dissolvedsubstances with the fluid surrounding them?

to avoid dilution of potential energy

to avoid osmotic imbalance and rapid gain or loss of water

to maintain the correct amount of dehydration and hydrolysis

4.What is entropy?

the amount of energy in a system

the amount of disorganization

the amount of organization

5.Burning a log is the reverse reaction found in photosynthesis.What products would be formed in burning a log?

carbon dioxide and water

glucose and oxygen

oxygen and carbon dioxide

oxygen and water

6.The main reason that cellular respiration needs to occur stepby step instead of a single, big reaction is

cells don't store enough oxygen

too much energy would be released for the cell to harness

cells don't have many mitochondria.

cells produce the enzymes needed for cellular respiration veryslowly

7.Enzymes are catalysts because they operate to:

raise activation energy

lower activation energy

supply activation energy

supply the reactants

8.When ATP powers a reaction it becomes ADP. The ADP

can reform ATP by an exergonic reaction

is a waste product for lysosomal breakdown

can reform ATP by an endergonic reaction

can reform ATP by an oxidation reaction

can reform ATP by an equilibrium reaction

9.The energy source that drives photosynthesis is

water

glucose

carbon dioxide

oxygen

sunlight

10. What is the process by which enzymes regulate their ownactivity?

osmosis

self-propulsion

feedback inhibition

11.Coupled reactions are reactions in which a

endergonic reaction drives a spontaneous reaction

exergonic reaction drives a spontaneous reaction

endergonic reaction drives an exergonic reaction

exergonic reaction drives an endergonic reaction

12.How is water used in photosynthesis?

as a substrate for ATP synthase

as an electron donor

to combine with ADP forming ATP

as an electron acceptor

13.Why are the âDark Reactionsâ of photosynthesis referred to asâDarkâ?

they do not require light

they can only occur at night

they generate excess sugar and the leaves turn brown

14.What is the difference between potential and kineticenergy?

potential is stored, kinetic is being used

potential is being used, kinetic is ready to be used

potential is negative, kinetic is positive

15.The products of cellular respiration are

carbon dioxide, water, and ATP

glucose, water, and ATP

glucose, carbon dioxide, and ATP

oxygen, ATP, and water

carbon dioxide, glucose, and water

16.All of the following are correct about energy except:

The biological world gets its energy from the sun.

Chemical bonds store potential energy.

A boulder perched on a hill has kinetic energy.

Energy is defined as the ability to do work.

All forms of energy can be converted to heat.

17.What is needed for the end product of glycolysis, pyruvate,to enter the citric acid cycle?

More glucose for energy

ATP

Oxygen

Carbon dioxide

18.The enzyme that forms a transmembrane channel in mitochondriaand phosphorylates ADP

a carrier protein

ATP synthase

acetyl CoA

19.Fermentation is most common in

plants

microorganisms

animals

20.During adsorption of sunlight by photosystems, H+ ions aregenerated. Where do they come from? What are they used for?

from the breakdown of sugar; they help form water

water; they help form sugar

from carbon dioxide; they help dissolve NaCl

21.Glycolysis

does not use oxygen

uses oxygen

uses carbon dioxide

22.Photosynthesis is

not dependent on chlorophyll

a process that produces glucose and oxygen

a process that produces water and carbon dioxide

23.Breakdown of glucose generates many ATP molecules, each ofwhich can be used independently. Why is ATP ideally suited to powerbiological reactions?

ATP has many hydrogen bonds

ATP has enough energy to power one biological reaction

ATP readily dissolves in water

24.Objects that are not actively moving but have the capacity todo so are said to possess:

kinetic energy

entropy

potential energy

living energy

25.The first stage of cellular respiration, called ___________,takes place in the cytoplasm of the cell and needs no oxygen.

photorespiration

citric acid cycle

glycolysis

oxidation

26.Diffusion

requires energy

utilizes proteins to move molecules across a membrane

moves molecules against a concentration gradient

does not require energy

cannot occur without a membrane present

27.What is the name of the molecule on which an enzymeworks?

substrate

monomer

polymer

28.ATP contains

phenylalanine

two phosphate groups

three nitrate groups

three phosphate groups

29.How does chlorophyll function in photosynthesis?

by absorbing carbon dioxide

by absorbing the sun's energy

by absorbing water

30.Objects that are moving are said to possess:

living energy

potential energy

entropy

kinetic energy

31.The respiration pathway that evolved first is probably

glycolysis

aerobic respiration

citric acid cycle

32.The energy source for the process of photosynthesis is

oxygen

sunlight

carbon dioxide

chlorophyll

glucose

33.Burning wood generates heat and light. What type of reactionis this?

exergonic

kinetic energy

endergonic

potential energy

equilibrium

34.What is meant by a âcoupled reactionâ in terms of energy?

many poymers are built simultaneously

energy released by one reactions powers another reaction

some reactions occur in the same organelle

35.The First Law of Thermodynamics states:

energy is constantly being created by entropy events

energy can be changed from one form to another, but cannot becreated or destroyed

energy can be used and then destroyed because of entropy'sactions on it

energy can be destroyed while it is producing entropy

36.How do we dispose of the carbon derived from the glucose thatis metabolized during respiration?

by breathing out

via our urine

it is broken down in lysosomes

37.Both plant and animal cells have mitochondria because theyboth

carry on respiration

carry on photosynthesis.

require water

38.What is Activation Energy?

the energy needed to initiate a reaction

the energy that transports water into cells

potential energy

39.If you put a plant in a chamber and shine a light on it

oxygen will decrease and carbon dioxide will increase

oxygen will increase and carbon dioxide will decrease

oxygen and carbon dioxide will increase

oxygen and carbon dioxide will decrease

40.What are the inputs and the output molecules for the overallphotosynthetic process?

input sugar and oxygen; output carbon dioxide and water

input oxygen and water; output sugar and carbon dioxide

input carbon dioxide and water;output sugar and oxygen

41.What kind of reaction is photosynthesis?

endergonic

kinetic energy

exergonic

potential energy

equilibrium

42.In the process of glycolysis, glucose is cleaved into a pairof three-carbon molecules called

lactate

pyruvate

acetyl CoA

ATP

alcohol

43.Entropy is

order

disorder

complexity

Both order and disorder are correct

Both complexity and disorder are correct

44.Glycolysis takes place in the _____________ and the citricacid cycle and electron transport chain take place in the___________.

cytoplasm; endoplasmic reticulum

mitochondria, chloroplast

mitochondria; cytoplasm

cytoplasm; mitochondria

45.The organelle that carries out photosynthesis in plants isthe

mitochondria

chloroplast

ribosome

chlorophylllysosome

46.The energy to power the Calvin cycle comes from

cellular respiration

oxygen

the light reactions of photosynthesis

47.Which of the following can be broken down into intermediateproducts that enter cellular respiration?

Proteins

Lipids

Carbohydrates

All of these.

48.When cellular product levels get too high, the responsibleenzyme is inhibited. This is an example of

promotion

denaturation

equilibrium

a coenzyme

feedback inhibition

49.How is respiration the opposite of photosynthesis?

respiration breaks sugar down to carbon dioxide; photosynthesisbuilds sugar from carbon dioxide

respiration builds sugar from carbon dioxide; photosynthesisbreaks sugar down to carbon dioxide

respiration requires energy input; photosynthesis gives offexcess energy

50.The molecule that absorbs sunlight for photosynthesis is

oxygen

carbon dioxide

chlorophyll

glucose

sunlight

amaranthbear841

3) The hydrolysis of sucrose to glucose and fructose is exergonic. However, if you dissolve sucrose in water and keep the solution overnight at room temperature, there is no detectable conversion to glucose and fructose. Why?

Select one:

a. The change in free energy of the reaction is negative.

b. The activation energy of the reaction is high.

c. The free energy of the products is higher than the free energy of the reactants.

d. This is a condensation reaction.

e. The change in free energy of the reaction is positive.

5) Competitive inhibition differs from non-competitive inhibition in that the ___1___ site is where we see competitive inhibition, while the non-competitive inhibitor __2___.

1. a. allosteric b. non-active c. active d. hydrophillic e. hydrophobic

2.
a. binds to the active site, thus blocking it
b. binds outside the active site, changing the shape of the enzyme
c. binds outside the active site, and blocks the active site

6) Phosphofructokinase is an enzyme, and as such it __1___ of the reaction. It is part of glycolysis, and is an example of an enzyme controlled by inhibition, as high levels of citrate (an intermediate further down the catabolic pathway) turns the enzyme off
(1)Select one:
a. lowers activation energy
b. lowers the ÎG
c. raises the Free Energy
(2)Select one
a. non-competitivenegative feedback
b. irreversible competitivereversible competitive.

15)The reactions in the pathways of glycolysis and the citric acid cycle harvest the high-energy electrons stored in glucose. Therefore, these pathways _______________.

Select one:

a. take place in animal cells, but not in plant cells
b. are catabolic pathways
c. are anabolic pathways
d. involve the reduction of glucose and its metabolic intermediates

21) In an exergonic chemical reaction, the products have less free energy than the reactants (-ÎG). Endergonic reactions require an input of energy (+ÎG). The addition of starting materials and the removal of end products prevent metabolism from reaching final equilibrium. Instead, the cell exists in a state of dynamic equilibrium.

Select one:
True
False

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BIOLOGY 2A03 Lecture Notes - Lecture 2: Uncoupling Protein, Intermembrane Space, Oxidative Phosphorylation

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