BIOLOGY 2B03 Lecture Notes - Lecture 4: Luciferase, Peroxisome, Signal Peptide
29 Jan 2016
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Document Summary
Major function of peroxisome is breaking of long fatty acids by beta-oxidation: in animals long fatty chains oxidized to medium chains then moves to mitochondria for further oxidation. Oxidation req for release of metabolic energy (both plants & H2o2-(catalase)->h2o +1/2o2 , the catalase in peroxisome breaks down the toxin into water and oxygen. Peroxisomes moves around the cell while changing shapes & undergoing fission & fusion. Peroxisomal proteins made in cytosol & transported to peroxisome. Peroxisomal membrane proteins ex:pmp70 are targeted to precursor membranes to create a peroxisomal ghost, once membrane proteins present, they transport peroxisomal matrix proteins ex: catalase into interior of the organelle. More proteins=growth and new peroxisome can form by fission. Rule 1 is there a peptide signal sequence? organelle: sig seq in luciferase is found on many peroxisomal proteins called pts1 (peroxisomal-transport sequence 1) Seq made of tripeptide: serine, lysine and leucine (skl seq)
