BCHM 102 Lecture Notes - Lecture 27: Adenine, Histone H2B, Deoxycytidine Triphosphate

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Lecture objectives: define the role of dna in storage of expression of genetic information, describe the structure of dna, understand the process of dna replication (synthesis, describe how eukaryotic dna is packaged into the cell. Transcription (rna synthesis: ge(cid:374)eti(cid:272) i(cid:374)fo(cid:396)(cid:373)atio(cid:374) f(cid:396)o(cid:373) dna is (cid:862)t(cid:396)a(cid:374)s(cid:272)(cid:396)i(cid:271)ed(cid:863) o(cid:396) s(cid:455)(cid:374)thesized i(cid:374)to (cid:373)esse(cid:374)ge(cid:396) na (mrna) Translation (protein synthesis: ge(cid:374)eti(cid:272) i(cid:374)fo(cid:396)(cid:373)atio(cid:374) f(cid:396)o(cid:373) (cid:373) na is (cid:862)t(cid:396)a(cid:374)slated(cid:863) o(cid:396) s(cid:455)(cid:374)thesized i(cid:374)to p(cid:396)otei(cid:374) Structure of dna: dna is a polyme(cid:396) of deo(cid:454)(cid:455)(cid:396)i(cid:271)o(cid:374)u(cid:272)lei(cid:272) (cid:373)o(cid:374)ophosphates (cid:894)dnmps(cid:895) (cid:272)o(cid:448)ale(cid:374)tl(cid:455) li(cid:374)ked (cid:271)(cid:455) 3(cid:859) 5(cid:859) phosphodiester bonds. Spatial orientation of 2 strands creates a major groove and a minor groove: provides access for binding of regulatory proteins. Cha(cid:396)gaff(cid:859)s ule: i(cid:374) a(cid:374)(cid:455) dou(cid:271)le st(cid:396)a(cid:374)ded dna (cid:373)ole(cid:272)ule: amount of adenine = amount of thymine, amount of cytosine = amount of guanine, a = t and c = g, amount of purines (a+g) = amount of pyrimidines (t+c)

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