MBB 231 Lecture Notes - Lecture 7: Integral Membrane Protein, Peripheral Membrane Protein, Isoprene

• School: Simon Fraser University
• Department: Molec Biol & Biochem
• Course: MBB 231
• Professor: Ingrid Northwood

Document Summary

Prof: dr. northwood: be clear on the three primary way proteins interact with membranes. 1: peripheral membrane proteins- non covalently bound to a membrane, lipid-anchored membrane proteins- covalently bound to lipid molecules. Functional distinction: distinguished by the conditions required to release. Addition of urea or carbonate: understand the difference between acetylated, prenylated and gpi anchored lipoproteins. Protein is synthesized in the cytosol and covalently attached to a saturated. Acetylated can be: fatty acid embedded within the membrane bilayer: myristolation-(14 carbons) attached to an amino terminal glycine, palmitolation- ( 16 carbons) attached to an internal cysteine or serine. Synthesized as soluble cytosol proteins before the adition od multiple 5- carbon isoprenyl groups in the form of: farsenyl group, geranylgeranyl group. Attached to the external surface of the plasma membrane are covalently. Green = sugar units that vary in number, nature and arrangement: know the four different types of integral membrane proteins. Type 1-single pass membrane protein, carboxyl end in the cytosol.