MBB 463 Lecture Notes - Lecture 12: Mutation, Daubert Standard, Repeat Unit
22 Jul 2020
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Learning objectices: define a complex dna profile, explain the characeristics of a complex dna profile, calculate peak height ratios, calculate lr and rmne for str, understand allele stacking, determine major and minor contributors. Order of interpretation of evidence: q (question) sample before k (known) sample, this is to avoid confirmation bias. Peak heights are a reflection of the true sample components: you want to identify alleles (the machine will determine this for you) and the genotypes to determine how many contributors are present in the sample, ex. Peak height ratios: used to determine heterozygote balance, sister alleles contribute equally to a mixture, so peak height ratios are always close to 1. Low peak height ratios can help you determine a second contributor: peak height ratios cam be different than 1 in single contributor profiles bc od pcr artecacts, 2 methods to determine it.
