CHEM564 Lecture Notes - Lecture 3: High-Performance Liquid Chromatography, Briey, Dextran

• School: University of Alberta
• Department: Chemistry
• Course: CHEM564
• Professor: Christopher Cairo

Document Summary

However, because proteins vary in size, charge, and structure, (compare to. Usually, protein mixtures are subjected to a series of separations, each based on a different property to yield a pure protein. Substantial quantities of puri ed proteins, of the order of many milligrams, are needed to fully elucidate their three-dimensional structures and their mechanisms of action. Thus, the overall yield is an important feature of a puri cation scheme. In this section, we brie y outline different techniques for separating proteins based on their physical properties. Generally speaking, if you are interested in purifying a protein then you already know its primary structure (the sequence of amino acids): from the sequence alone, you can calculate some properties which can help guide the puri cation procedure. However, these tools do not account for the fact that the protein has a 3-dimensional structure and only calculates properties that are independent of structure. http://ca. expasy. org/tools/#primary.