BIOL 331 Lecture Notes - Lecture 34: Cyclin-Dependent Kinase 1, Protein Kinase, Wee1
3 Apr 2018
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Document Summary
Yeast cells have a single cdk, however, mammalian cells produce several versions of this kinase. Different cyclin-cdk complexes target different substrates at different points in the cell cycle. A model of cell cycle regulation in yeast (on right) Cdk/cdc2 kinase activity correlates with protein level. Protein level is shown as the two curves (purple and blue) The peaks of the curves is when protein synthesis occurs. Progression through the yeast cell cycle requires phosphorylation and. Essentiall phosphorylation is done by cyclin activating kinase (cak), and it occurs in threonine 161 (thr161) Inhibitory phosphorylation of tyrosine 15 (tyr15) by wee1 (type of protein kinase) until end of. Tyrosine 15 is dephosphorylated by cdc2, activating tyr15. Tyr15 is inactive as long as the additional p is attached, so to activate it, you need a phosphatase to remove that additional p thereby making tyr15 active.
