1. for a protein, P, which has a hyperbolic binding curve for a ligand, L, (like Michaelis-Menton enzyme), choose a dissociation constant in the nano- or micromolar range. Choose a series of ligand concentrations, and plot the fraction of sites occupied on the Y axis against the ligand concentration on X. Be sure the ligand concentrations are chosen so that all or nearly all the binding curve is shown in your plot.
