BIOL 2500 Lecture Notes - Lecture 15: Autoradiograph, Replisome, Dna Replication
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20 May 2016
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The integrity of the nucleotide sequence of dna is of paramount important. The general mechanism of dna replication is the same in all organisms. As organisms diverged and became more complex, some differences did develop in the replication proteins and enzymes. Dna replication is most often bidirectional, proceeding in both directions from a single origin of replication in bacterial chromosomes. Eukaryotic chromosomes have multiple origins of replication. Autoradiograph analysis shows multiple origins of replication on eukaryotic chromosomes. Large eukaryotic genomes contain thousands of origins of replication separated by 40,000 to. The human genome contains more than 10,000 origins. Dna replication rate varies among different types of cells. Though relation is very similar among bacteria, archaea and eukarya, the processes are not identical. The enzymes and proteins involved are parts of large complex aggregations of proteins and enzymes called replisomes. Replication origins have sequences that attract replication enzymes.
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