BIOL308 Lecture Notes - Lecture 17: Leucine Zipper, Basic Helix-Loop-Helix, Homeosis

• School: University of Waterloo
• Department: Biology
• Course: BIOL308
• Professor: John Heikkila

Document Summary

Note: included parts from previous lecture for context and continuation of list. 1: a helix in bzip (basic leu zipper) 2: a helix in bhlh (basic helix loop helix) 4: basic helix loop helix (bhlh: 2 a helices connected by non-helical loop, n terminal is positive and has basic aas in the dna bd, c terminal has hydrophobic residue dimers or heterodimers. Dimer type 1: non basic monomer hlh proteins bind together and can"t bind dna or interact with the response element therefore there will be no functional activator binding which will result in less/no expression. Dimer type 2: bhlh proteins dimerize and bind dna which forms a functional activator protein which results in expression. Transcription activation domain structural motifs: acidic (glu/pro rich) with b sheets, structures vary due to protein: protein interactions and contacts/activations, there is independence of dna bd (for binding only) and transcription activation domains (for activation only)