BIOL 200 Lecture Notes - Lecture 5: Biological Membrane, Chromatin, Unit
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Resolutio(cid:374) is good, (cid:272)a(cid:374) see (cid:374)u(cid:272)lear e(cid:374)velope, thi(cid:374)gs that you (cid:272)a(cid:374)"t see i(cid:374) light microscopy. Cell surface receptors for signalling growth, activation. Orientation of membrane components maintained during vesicle budding and fusion. Two sides: cytoplasmic-facing, non-cytoplasmic facing (which describes the lumens of er, golgi, vesicles, extracellular fluid) Agp4 after photo bleached there is little change to the membrane protein. Gpa1 after photo bleached, protein is dissipated. Membrane carrying protein agp4 has circular bleached area between panels a+c (before and after bleaching) not much difference in fluorescence of the spot. If it"s e, the(cid:374) you assu(cid:373)ing the two membranes are different. (wrong) Data is in absolute terms agp4 = not mobile, gpa1 mobile. Gpa1 diffuses more quickly through the membrane then agp4. Gpa1 could be a peripheral protein because it has weaker non-covalent bonds than. Agp4, which is possibly a integral proteins with strong non-covalent bonds.
