BIO 4115 Lecture Notes - Lecture 14: Holliday Junction, Cytidine, Crispr
12 Apr 2020
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Ku dimer dna pkes ligate ends quick terror. Trac rrna pre crrn at cas9: scan for pam. Hdr proteincomplex w rad 50 see break resection initiate pairing wl homo region. Foregin dna invade bacteriafragment into 2ont e insert into crispr locus w short repeats pre crrna transcribe tracerrna casa bindprecrrna tracrrnat casatpre crrnaprocessed by rnase111 to mature ind units. Nts bind dna spacerbindpam cleave 3rd nt after pam. Pam on opp strand 355: cleave 3rd nt after. H840a rwc cleaveopp1553 d casa fuse w heterologous effect domain methyl silent. Uridine t based on cytosinedeamin ssdna specificcytidinedeamin grna. Cytidine1adenosine deaminase bind a base on strand 0g rna bound. Prime editing targeted indels et base is wio dsdna break. Reverse transcriptase pegr na t h 840a chick wl pam. 3 end wi primerbinding site bind pam rt replicateseqpegrna donor dnatemplate. Seetadsorganized in compartments domains can sep reg elements self interaction w. Dox bind tre turn on cmvwhich activate cfp.
