BIO206H5 Lecture Notes - Lecture 10: Inosine, Uracil, Stem-Loop
Document Summary
Spliceosome made up of 5 different snurps. Needs to be able to recognise intron and exon junction - u1. 3d structure of intron is modified so that it approaches 5" end. This bending that happens is what causes branch-point adenine to become a catalytically active one. A specific protein identifies a particular site. Branch-point a is identified by a branch-point binding protein. It gets shoved out of the strand, while the remainder is base-paired. Bulging moves branch-point adenine so that the adenine can catalytically act. Introns allow for a single gene to code for a large number of different proteins. 5" cap is an indication that the rna molecule has been full processed. A number of other proteins are associated with the mrna molecule. Protein synthesis itself has a directionality to it. Rna is translated from 5; end to 3" end. There is a start translation signal and a stop translation signal.
