BIO206H5 Lecture Notes - Lecture 7: Zinc Finger, Polyadenylation, Covalent Bond
Document Summary
Know structure of amino acids, of nucleotides, names and abbreviations of amino acids. During initiation phase, rna polymerase has an extra subunit (sigma factor). Sigma 70 is a sigma factor involved in transcribing genes involved in housekeeping (general everyday operation of biochem of that cell). Alpha: recognize upstream subunits (-40 to -70) beta: catalytic activity. When the protein binds, it has directionality to it. It points to a certain direction (direction transcription will occur in). It twists and bends it into a very distorted shape. Closed complex is in the core of the protein it cannot be transcribed like this. There needs to be a rxn that pries that region open. This gives us the notion that it"s a vectoral enzyme. This also helps in terms of defining which is the template/non-template strand. The non-template strand tells you what the nucleotide sequence for. This is why we write the non-template strand bc not only does it tell.
