BIO206H5 Lecture Notes - Lecture 9: Rna Splicing, Ribonucleoprotein, Intron
Document Summary
There are many chromatin proteins to help unwind the dna as the polymerase is going through, heavily complex with histone proteins. One of the problems with eukaryotic system is that it is very complicated- the gene itself has intervening sequences called introns which have to be removed. Rna sequence elements allow for intron-exon junction to be clearly identified. 5" gu- these gu are conserved, on the other end just before the 3" splice site, the ag are completely conserved. These are sufficient for the identification of the introns by the splicing complex. Branch point adenine- it is slightly upstream of the pyrimidine rich region. The diagram that is titled rna splicing rxn mechanism is not be seen as two strands of dna. Spliceosome complex is what essentially catalyzes the series of rxns. It is made up of macromolecule protein and rna super-complex, an assembly of 5 snurps.
