BIOB11H3 Lecture Notes - Lecture 6: Southern Blot, Northern Blot, Paper Towel
Document Summary
Tom + self-splicing (figure 11-31: lower eukaryotes have group 1 introns, group 2 introns used a cowboy lasso looking structure (lariat) Loops, partial double stranded character = stems are close due to complementary base pairing. Self-splicing rna has internal complementarily guides it +intron sequences whereas spliceosomes are dependent on snrna molecules + base pair + activates a = cleavage and ligation similar mechanism. Figure 11-34 = 5" cap recognizes 5" end, binding sites on tail for cap possible/ spliceosome + capping and happened before rna finishes its job. Figure 11-35 = introns yellow, exons blue, rna isolated from nucleus vs. cytoplasm, notice lower molecular weight bands in association with hybridization (keep getting shorter), band size decreases. Figure 11-38 =3 different zebrafish embryos: take mirnas and link them to blue colours, light up different body parts, proves that they are involved in diff. processes and tells us where are processed.
