BIOC17H3 Lecture Notes - Lecture 4: Petri Dish, Norman Pace, Microbial Ecology
26 Mar 2020
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Culture independent methods confirmed the crisis and solved the problem: Microbial ecology and evolution: a ribosomal rna approach. Olsen gj, lane dj, giovannoni sj, pace nr, stahl da. Isolation of microbial 16s rrna genes directly from the environmentfew samples from extreme environments yield more diversity than the cultivated microbes from the entire world . Bioc17: what did they do: extract all dna from an env. Sample/microbial community use pcr and primers to amplify the 16s rna, amplified all the rna found what types of them are viable in these env. Bioc17: comparative analysis of 16s rrna gene from thousands of species, demonstrated the presence of signature sequences (short conserved sequences that can be highly diagnostic of group of related organisms) Bioc17: domain is equivalent to phylum, 1994 13 divisions, all cultured non are uncultured, 1997 37 divisions. The public perception of microorganisms: large organisms: more diverse, numerous and complex than microbes.
