BIO230H1 Lecture Notes - Lecture 4: Ubiquitin Ligase, Tumor Suppressor Gene, Plant Hormone
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BIO230H1 Full Course Notes
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Document Summary
Regulation of the proteome: shine-dalgarno sequence, eukaryotic regulation, protein folding and polyubiquitin, post-translational protein mods. Both proks and euks use to reg expression in resp to stressful situations. Prokaryotes mrnas have six nucleotide shine-dalgarno (sd) sequences upstream of aug start codon. Correctly positions aug in ribosome and provides translational control mechs. If sd sequence missing or blocked, translation will not happen. Below: no cap or poly-a tail so it is prokaryotes. Lower temperature creates conformational change to form hydrogen bonds. Rna made elsewhere that binds to the sequence. No sd seq but similar mechanisms as proks. Translational repressors can bind near aug and inhibit translations (eg. aconitase) Aconistase binds near the start site and blocks translation. Aconitase binds iron to cause a conformational change. Can use repressor proteins to interfere with 5" cap and 3" poly-a which stops translation. Small rna molecules can reg translation (mirnas) - diff mechanism than prokaryotes.
