BIO230H1 Lecture 1: BIO220 Exam REVIEW

Part C, General Science (questions 34-40)

34.If the electrical charge inside a cell was -70 millivolts,how many volts would this be?

a.-0.7 volts b. -0.07 volts c.-7.0 volts d. -70,000 volts e. -70 volts

35. What is the pH of solution that has a concentration of H+that is 0.001 moles/liter (hint: express this number usingscientific notation, then use the equation. pH = - log [H+].

a. -1 b. 3 c. 13 d. 7 e. -3

36. Using the graph below, with a ventricular stretch of 200 ml,what would be the stroke volume?

a.50 ml b. 70 ml c. 90 ml d. 110 ml e. 200 ml

37. If your heart pumps out 70 ml of blood per beat (strokevolume) and your heart rate is 60 beats per minute, what is theoutput of your heart in ml per minute (this is your cardiacoutput)

a.10 ml

b. 420 ml

c. 4,200 ml

d. 420 liters

e. 2.1 liters

38. 3,500 excess calories will eventually be converted to 1pound of fat tissue. If you consume 70 calories a day more than youburn, how long would it take you to gain one pound of fat

a. 5 days b. 2 weeks c. 50 days d. 2 months e. 5 months

39. Following rigorous exercise, your heart rate was 40 beats in20 seconds. What is your heart rate in beats per minute?

a.80 b. 160 c. 100 d. 90 e. 120

40. The amount that you learn in physiology is positivelycorrelate with

a. the frequency of your attendance

b. your desire to engage in lectures and labs

c. your asking questions when you don’t understandsomething

d. your willingness to address and work on youracademic weaknesses

e. all of these

1. The biggest single risk factor for cancer is

a. age

b. environmental mutagens and carcinogens

c. sunlight

d. tobacco

e. all of the above are about equal.

2. True or False: Cancer occurs more frequently in epithelialtissues than in connective tissues

3. True or False: Many different cancers often have mutations inthe same

4. Senescent cells

a. Are normally smaller than ordinary cells

b. Are metabolically inactive

c. Are removed from the cell cycle

d. Are known to prevent cancers from arising

e. All of the above

5. The most common mutation in cancer is a mutation to the genenamed _____

6. The protein ubiquitin is attached to other proteins byubiquitin ligase in order to______________________________________________________________.

7. Some RNA viruses are able to insert their genomes into host(human) cells by use of the enzyme____________________________________________

8. Many scientists believe that telomere attrition is ananti-cancer mechanism used by the body to ________________ cell____________________.

1. Why is MgCl2 included in the PCR buffer? MgCl2 is used in the PCR buffer because it help 2. If you started a PCR protocol with 10 double stranded molecular of DNA, how many double stranded molecules of product would you have after 5 cycles of PCR? 3. Where is the initiation and stop codon of the GFPuv gene in the forward and reverse primers, respectively? 4. What kinds of materials obtained from a crime scene might contain DNA, and where might you find them at a crime scene? 5. Why do you need to perform PCR on DNA obtained froma crime scene? 6. What is genotype? 7. What is the different between an allele and a locus? 8. What is an allele ladder? What is its function in DNA profiling? 9. Why do forensic labs analyze non-coding DNA and not genes?\ 10. How unit is defined for the restriction enzymes EcoRI and HindIII from New England BioLabs? 11. What is the star activity of restriction enzymes?