CSB346H1 Lecture Notes - Lecture 5: Control Of Ventilation, Muscle Tone, Sleep Paralysis
Document Summary
We talked about the experiment where you manipulate the pre-b tc in an anesthetized rat. Other people wanted paul to do the same experiments, but in awake animals: scientists kill rats by stopping them from breathing while awake. this was the single handed problem with these experiments. The idea of this experiment was a problem. The hypothesis is that if you disrupt the pre-b tc, it should stop normal breathing (or eupnea). This is about how they achieved these goals. This explains how they can shut off pre-b tc cells temporarily to show that they are responsible for generating respiration. The allatostatin receptor is a bug receptor, hence bugging . They used an aav2, which drives the expression of alstr in pre-b tc cells. When you give allatostatin, it allows you to open up the receptor. When it opens up, k+ flows out. There is a lot of k+ inside the cell.
