IMM340H1 Lecture Notes - Lecture 13: Histone H2B, Hapten, B Cell
Document Summary
The hapten is being used for very specific regions. We can have the tcr recognize something that the. Bcr cant, and we can either take the 2 pieces of the antigen apart, or put them together. In a 2nd culture we put dnp linked to f. i(cid:374) this situatio(cid:374), (cid:449)e do(cid:374)"t get a pla(cid:395)uea(cid:374)d thus the(cid:396)es (cid:374)o t (cid:272)ell activation. The dnp needs to be covalently linked to whatever the t cell is recognizing. The(cid:455) do(cid:374)"t (cid:396)e(cid:272)og(cid:374)ize klh, the(cid:455) e(cid:272)og(cid:374)ize a peptide de(cid:396)i(cid:448)ed f(cid:396)o(cid:373) klh. We takr this t cell clone and mix it with singenaic b (cid:272)ells, (cid:272)ells that e(cid:454)p(cid:396)ess h2b, a(cid:374)d (cid:272)ells that do(cid:374)"te(cid:454)press h2b. In the mixture of cells with antigen, do we see dnp specific plaque forming cells and we know there is because the b cells get activated. When we plate with h2b, we see plaques. After killing with h2d, theres only b cells left and theres no plaques.
