IMM340H1 Lecture Notes - Lecture 2: Inbred Strain, Mhc Class I, Backcrossing

76 views17 pages

sangriacrocodile19

9 Feb 2018

School

Department

Course

Professor

For unlimited access to Class Notes, a Class+ subscription is required.

Document Summary

Question #1: well we now ask if different mhc alleles expressed by the apcs can restrict the t cells" function, does the mhc locus expressed on the apc restrict the function of the. Mouse have 4 mhc loci and you need to memorize that k d is for mhc class i, ia, ie is for. Mouse are diploid organisms, so they will carry two alleles per locus. For the inbred strain, because they are inbred, they are homozygous, carry the same alleles on these 4 loci, while the. You only need 1 locus product k or d, ia or ie to get either mhc class i and ii respectively. But there is a defect for the e alpha chain, so the mhc class ii from i-e in a h- In f1, you will see it is hemizygous on i-e. So we know there are two loci products that can give rise to mhc class i: k and d. (can ignore.

Related Questions

Immunology worksheet help?

In people infected with human cytomegalovirus, class I MHC andb2-microglobulin are produced, but very little mature class I MHCis found at the cell surface. Inhibition of which of the followingmolecules by human cytomegalovirus may account for thisphenomenon?

A.	TAP
B.	Ii
C.	TCR
D.	CLIP
E.	RAG

Your chances of having the same MHC haplotype as your siblingare:

A.	1:2.
B.	1:4.
C.	1:16.
D.	1:8.
E.	1:1.

Which of the following is the BEST example of self-MHCrestriction of T cells?

A.	A T-cell clone is deleted when it binds too tightly to selfMHC.
B.	A T-cell clone responds more vigorously to a self-peptide thana foreign peptide.
C.	A T-cell clone recognizes foreign MHC.
D.	A T-cell clone only recognizes foreign peptides when presentedby self MHC.
E.	All of the above

MHC molecules:

A.	are conserved between individuals.
B.	bind repeating patterns found on pathogens.
C.	are recognized by T cells.
D.	are membrane-bound molecules found exclusively on T cells.
E.	are secreted by T cells and bind and neutralize antigen in theserum.

Tasmanian devils are a largely inbred population with littlediversity in the MHC. Which of the following would be a likelyoutcome of this?

A.	They would compensate for low diversity in the MHC with highdiversity in the TCR.
B.	They would compensate for low diversity in the MHC with highdiversity in the BCR.
C.	They would rely only on innate immune responses.
D.	They would be at higher risk of infection.
E.	All of the above

How many different MHC classical class I proteins does eachnucleated cell in a human heterozygous at the MHC locusexpress?

A.	Six
B.	Two
C.	Three
D.	One
E.	None. Only APC express class I.

MHC polymorphisms tend to cluster:

A.	in the peptide-binding groove.
B.	in the transmembrane domain.
C.	in the coreceptor contact residues.
D.	in the framework region.
E.	All of the above

A mutation that renders the MHC class II molecule DMnonfunctional would likely lead to:

A.	cells with no class II MHC.
B.	a deficiency in CD8⁺ T cells.
C.	cells with class II MHC only bound with CLIP.
D.	cells with class II MHC bound with a variety ofself-antigens.
E.	cells with no DO.

The human MHC locus is one of the most polymorphic regions ofthe genome. What does this mean?

A.	This region is regulated by many different mechanisms.
B.	Sequence variability is low in this region.
C.	There are many introns in this region.
D.	There are many different alleles of these genes.
E.	It has the most different genes.

10.

Which of the following is an example ofcross-presentation?

A.	An exogenous peptide being presented on MHC class II
B.	A B-cell antigen being presented to a T cell
C.	An endogenous peptide being presented on MHC class I
D.	An endogenous peptide being presented on MHC class II
E.	An exogenous peptide being presented on MHC class I

magentacamel430

Question XV. The genes encoding MHC class I and classII molecules are highly polymorphic in human populations. SpecificMHC allotypes have been associated with increased susceptibility orresistance to certain viral infections. This influence of MHCmolecules on disease susceptibility can be best explained by thefact that [complete the sentence and assess each subsequentstatement (135-138) as either factually correct (= âtrueâ) orincorrect (= âfalseâ)]:

This question has confused me. The use of the word "best" seemsto imply there is one answer that is true and all others are false.Please answer this question in the context that 1. you could onlypick ONE answer as the BEST, which one you would pick and 2. if youcould answer more than one as true/best which ones would betrue

135. viral infection of DCs results in the presentation of viralpeptides by MHC molecules, which stimulates the activation of naiveT lymphocytes.
136. T cells become restricted to either MHC class I or MHC classII allotypes during their thymic development and this determineswhether they become polarized towards TH1 or TH2 responses,respectively.
137. different MHC allotypes differ in their ability to distinguishharmful compounds from harmless self antigens.
138. different MHC allotypes bind different peptides and,therefore, some allotypes can present peptides from certain virusesbetter than peptides from other viruses.

amethystcod591

Question 4 (9 points):

To study T cell development, and self-restriction of T cells, you decide to perform some experiments. You obtain four mouse strains: B, B-MCC, K, and K-MCC. B strain mice express MHC molecules of the b haplotype (e.g. H-2K^b, H-2D^b, I-A^b) while K mice express MHC of the k haplotypes (e.g. H-2K^k, H-2D^k, I-A^k). The strains are otherwise genetically identical. B-MCC and K-MCC are identical to the B and K strains (respectively) excepts that contain a transgene encoding the antigen Moth Cytochrome which is not present in normal mice. MCC is produced as both a secreted and cytoplasmic protein and it is expressed by dendritic cells and thymic epithelial cells. You lethally irradiate groups of mice from all four strains and perform all possible bone marrow transfers among the mice. In other words, the BM of each strain is transplanted into mice of each strain, producing 16 different combinations of donor BM and recipient strain. You now take T cells from the lymph nodes of each of the 16 types of transplanted mice and test their ability to proliferate in response to MCC-presenting B cells that express only class II MHC molecule, I-A^k. Fill out the chart below by indicated which mice will have T cells that respond. Use (+) to indicated proliferation and NR to indicate a lack of proliferation. In addition, for the mice labelled a-e give a brief explanation for your answer. Include the mechanism of T cell selection experienced in thymus (positive selection, negative selection, or neglect) and the cells involved in selection (thymic epithelial cels, BM-derived APCs, or both).

Table (4 points, 0.25 points per box)

Recipient Strain Â®

BM Donor	B	B-MCC	K	K-MCC
B			c.
B-MCC			d.
K	a.	b.	e.
K-MCC

1 point for each explanation

yellowguinea-pig868

IMM340H1 Lecture Notes - Lecture 2: Inbred Strain, Mhc Class I, Backcrossing

Document Summary

Get access

Related Documents

IMM340H1 Lecture Notes - Lecture 6: Paratype, Immunoglobulin G, Thymocyte

IMM340H1 Lecture Notes - Lecture 11: Il-2 Receptor, Syngenic, Interleukin 2

IMM340H1 Lecture Notes - Lecture 3: Keyhole Limpet Hemocyanin, Virus Quantification, Mhc Class Ii

Related Questions