Anatomy and Cell Biology 3309 Lecture Notes - Lecture 1: Collagen, Basophilic, Nanometre

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The study of structure and function of cells, tissues and organs at the microscopic level in order to understand normal function and to diagnose pathology. Tissues are building blocks that all organs and organ systems are made of. 4 components all living things are made of (chemical composition of tissues): sugars, nucleic acids, proteins, lipids. The biochemical nature of sugars, nucleic acids, proteins and lipids allows them to be distinguished with different agents that can be applied: can differentiate the different components to visualize the different structures in the tissue. Traditional light microscopy (cid:523)(cid:494)bright field(cid:495)(cid:524) of stained tissue sections: uses visible light to illuminate structures. Phase or differential interference contrast (nomarski): uses different refractive indices within a specimen for visualization of living cells in culture. Electron microscopy: uses an electron beam to visualize differences in material density in very small samples: study fine structures and detail. Light is focused by a condenser lens onto the specimen slide.

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