Biochemistry 2280A Lecture Notes - Lecture 33: Dna Profiling, Gene Knockout, Rna Virus
12 Dec 2016
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The chains would never stop and no sequence could be read. The chains wouldn"t terminate so you"d synthesize the full size of dna which wouldn"t make bands so it would just show at the very top of the gel. C. the probability of stopping the chain would increase and few bases would be read. This is if you have too much dideoxy would make it terminate early: in the mid 1990s, automated dna sequencing became common place. Each ddntp is labeled with a different dye that fluoresces at a different wavelength you just read the sequence from the different fluorescent patterns: expressing yfg in e. coli. B. introns are found in human genes. Introns are dealt with at the beginning because we use cdna rather than genomic dna. C. they differ in their mechanism of translational initiation. Eukaryotic scan from 5" end and read from the first aug. Bacterial ribosome starts from the scanning site.
