Biochemistry 2280A Lecture Notes - Lecture 27: Plant Breeding, Styrofoam, Synthetic Biology
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Accurately identifying the start site of translation is essential to make sure the correct protein is made. Starting a protein out-of-frame results in a nonfunctional protein: eukaryote translation initiation. We start at a specific initiator met trna binds to the small ribosome subunit forms complex. Subunit complex recognizes the 5" cap on the eukaryotic mrna (without. Aminoacyl-trna comes in translation happens in the cytosol. It might decrease translation the hairpin would stop the small subunit from recognizing the aug stops the scanning and prevents subunit from ever getting to the aug. C. it might increase translation: q: why must translational initiation in bacteria use a mechanism other than 5" scanning? (prokaryotes) There are no small ribosomal subunits in bacteria. There are multiple proteins encoded from common mrnas in bacteria related to operon structure. If ribosomes used a scanning mechanism, the only protein that would get translated would be the first aug. There"s internal genes in the operon structure.