Biochemistry 2280A Lecture Notes - Lecture 23: Mass Spectrometry, Ribosomal Rna, Transfer Rna
1 Jan 2017
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When human genome was first released, it was done by 2 teams: craig venter, francis. Estimated to be a billion business. Traditional genome sequencing involves: create a genomic dna library cloning, many independent sequence reactions, aligning the independent sequences into a continuous sequence. Collection of cloned dna fragments that represents all of the dna in an organism"s genome. Have any copies of human genomic dna. Cleave it with a restriction enzyme (partial digest) to get fragment. Millions of fragments because genome size is so big. Do cloning: ligate fragments into vector, transform into e. coli, get millions of colonies. Cloning using genomic dna as the insert. Independent clones that combine to represent the whole human genome in small fragment. Genome: circular like e. coli (smaller, 2 million bp in size. Clicker q: the h. fly genome is 2 million base pairs.
