Biochemistry 2280A Lecture Notes - Lecture 15: Okazaki Fragments, Dna Replication, Helicase
15 Jan 2017
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Document Summary
New strand is made from a template of the old strand. In the new double helix, one strand is old (conserved) and one strand is new. Bacteria have conserved location for their replication origin circular genome has only 1 origin , Eukaryotes have much much larger genomes and divided into multiple linear chromosomes. At base pair is less stable than gc base pairs. Replication origin is recognized by specific initiator proteins. However, complementary nature of the stands makes it want to return to the double. Role is to find replication origin and separate dna to prep it for replication stranded state or form secondary structure with itself (ssbp) Re-annealing is prevented by the binding of single-stranded binding proteins. Sspp does not bind tightly - dna-polymerase can just push it off. Helicases bind at each of the replication fork (each side of the bubble) Job is to extend the single strandedness of dna: priming.
