Biochemistry 2280A Lecture Notes - Lecture 14: Rna Splicing, Phosphodiester Bond, Polynucleotide

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Learning objectives: explain the chemical structure of nucleic acid polymers, without memorizing the structures of the nitrogenous bases, describe b-dna and the higher order structures formed by rna, and the important forces that stabilize these structures. Dna holds genetic info passed from parent(s) to offspring that is required to produce an organism. Rna and dna are based on five carbon sugars! Purines contain a five-membered ring and six-membered ring joined together. Nucleosides contain a ribose and nitrogenous base, but no phosphate groups. Phosphates are normally added to the 5" carbon of the sugar. Up to three phosphates are added in a linear fashion, joined by phosphoanhydride bonds. Polynucleotides are assumed to start at the 5" end when written. 2" position in a sugar ring is not normally involved in a phosphodiester bond in a polynucleotide: in dna, this position doesn"t even have a hydroxyl group, making it relatively chemically inert, yet dna can form polynucleotides.

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